| Amount (μl) | Final concentration | |
| Q5® High-Fidelity 2X master mix | 25 | 1 × |
| sgRNA cassette forward (10 μM) | 2.5 | 0.5 μM |
| sgRNA cassette reverse (10 μM) | 2.5 | 0.5 μM |
| Template gBlocksTM | 100 ng | < 1,000 ng |
| Nuclease-free water | Add up to 50 | |
| Final volume | 50 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.