| Each well (six-well plate) | Each well (12-well plate) | |
| CasRx plasmid DNA (ng) | 1,600 | 800 |
| VEGFA sgRNA gBlocksTM (ng) | 200 | 100 |
| Lipofectamine 2000 (μl) [DNA (μg)/Lipofectamine 2000 (μl) = 1:3] | 5.4 | 2.7 |
| Opti-MEM (μl) | 300 | 100 |
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