| Each well (six-well plate) | Each well (12-well plate) | |
| CasRx plasmid (ng) | 800 | 400 |
| VEGFA pre-sgRNA plasmid (ng) | 800 | 400 |
| Lipofectamine 2000 (μl) [DNA (μg)/Lipofectamine 2000 (μl) = 1:3] | 4.8 | 2.4 |
| Opti-MEM (μl) | 300 | 100 |
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