| Each well (six-well plate) | Each well (12-well plate) | |
| All-in-one pAAV–CasRx–pre-sgRNA plasmid (ng) | 1,600 | 800 |
| ViaFect (μl) [DNA (μg)/ViaFect (μl) = 1:6] | 9.6 | 4.8 |
| OptiMEM (μl) | 300 | 100 |
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