. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879
Copyright © 2021 Chuang, Wang, Kumar, Lama, Lin and Liu.
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| Temperature (°C) | Time (min) | |
| Step 1 | 25 | 10 |
| Step 2 | 37 | 120 |
| Step 3 | 85 | 5 |
| Step 4 | 4 | ∞ |