| Component | Amount/reaction (μl) |
| TaqMan Fast Advanced Master Mix | 5 |
| TaqMan primer (GAPDH, dilute with DNase-free water in a ratio of 1:3) | 0.5 |
| TaqMan primer (human VEGFA) | 0.5 |
| Nuclease-free water | 2 |
| Final volume | 8 |
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