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. 2021 Jun 11;9:667879. doi: 10.3389/fcell.2021.667879
Component Amount/reaction (μl)
Top oligonucleotides (100 μM) 1 (5 μM)
Bottom oligonucleotides (100 μM) 1 (5 μM)
ATP (25 mM) T4 polynucleotide kinase (PNK) PNK buffer (10 × ) 2 (2.5 mM) 1 (10 U) 2
Nuclease-free water 13
Final volume 20