Figure 3.

Supplementation with inulin or sodium butyrate induces Defa5 protein expression in ileal Paneth cells. C57BL/6 mice fed different diets as described in Figure 1 are shown. (A) Representative images of Defa5 immunofluorescence staining in ileal tissue showing Defa5 expression in green and cell nuclei detected by DAPI in blue. Scale bar: 100 µm. (B) Quantification of Defa5 expression. Data are presented as means +/- standard error of the mean (SEM). Statistical analysis was performed by one-way ANOVA with Dunnett´s or Sidak´s post-test. Significant differences are indicated as **p-value < 0.01; ***p-value < 0.001.