Figure 8.

Sodium butyrate induces expression of antimicrobial peptides in murine organoids in vitro. Organoids were treated with 1mM and 2mM sodium butyrate for 30h. Relative mRNA expression level of cryptdin-1 (A), cryptdin-4 (B), pan-cryptdin (C), MMP7 (D), lysozyme (E) Reg3γ (F), mBD-1 (G) determined by quantitative RT-PCR derived in organoids from the small intestine of healthy C57BL/6 mice (n=6). Organoids were treated with sodium butyrate (1mM), sodium butyrate in combination with HJC0152 (5μM) or MS-275 (2μM) or HDAC8-IN-1 (0.5μM). Relative mRNA expression level of cryptdin-1 (H) and cryptdin-4 (I) determined by quantitative RT-PCR derived in organoids from the small intestine of healthy C57BL/6 mice (n=3-6). Data are presented as means ± SEM and were analyzed by unpaired t-test (A–G) or one-way ANOVA with Sidak´s post-test (H, I). Significant differences are indicated as *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001.