Figure 9.

Effect of sodium propionate and acetate on expression of antimicrobial peptides in murine organoids in vitro. Organoids were treated with sodium propionate (1mM) or sodium acetate (1mM) for 30h. Relative mRNA expression level of cryptdin-1 (A), cryptdin-4 (B), pan-cryptdin (C), MMP7 (D), lysozyme (E) Reg3γ (F), mBD-1 (G) determined by quantitative RT-PCR derived in organoids from the small intestine of healthy C57BL/6 mice (n=3-6). Data are presented as means ± SEM and were analyzed by unpaired t-test. Significant differences are indicated as *p-value < 0.05; **p-value < 0.01; ***p-value < 0.001.