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. 2021 Jun 11;12:678360. doi: 10.3389/fimmu.2021.678360

Table 1.

Primers used in quantitative real-time PCR.

Forward (5’-3’) Reverse (5’-3’)
Actb GCTGAGAGGGAAATCGTGCGTG CCAGGGAGGAAGAGGATGCGG
Defa1 TCAAGAGGCTGCAAAGGAAGAGAAC TGGTCTCCATGTTCAGCGACAGC
Defa21 CCAGGGGAAGATGACCAGGCT TGCAGCGACGATTTCTACAAAGGC
Defa29 CACCACCCAAGCTCCAAATACACAG ATCGTGAGGACCAAAAGCAAATGG
pan-cryptdin AAGAGACTAAAACTGAGGAGCAGC GGTGATCATCAGACCCCAGCATCAGT
Mmp7 TTCAAGAGGGTTAGTTGGGGGACTG CCGCCTCTACGAGTGAAACTGTT
Lyz1 GCCAAGGTCTACAATCGTTGTGAGTTG CAGTCAGCCAGCTTGACACCACG
Nod2 GGCACCTGAAGTTGACATTTTGC ATCTCCCACAGAGTTGTAATCC
Reg3g TTCCTGTCCTCCATGATCAAAA CATCCACCTCTGTTGGGTTCA
Myd88 CAAAAGTGGGGTGCCTTTGC AAATCCACAGTGCCCCCAGA
Defb1 TCCAATAACATGCATGACCA TCATGGAGGAGCAAATTCTG
Ocln ACTCCTCCAATGGACAAGTG CCCCACCTGTCGTGTAGTCT
Cldn2 GTCATCGCCCATCAGAAGAT ACTGTTGGACAGGGAACCAG
Cldn5 GCTCTCAGAGTCCGTTGACC CTGCCCTTTCAGGTTAGCAG
ZO-1 CCACCTCTGTCCAGCTCTTC CACCGGAGTGATGGTTTTCT
Muc2 GATGGCACCTACCTCGTTGT GTCCTGGCACTTGTTGGAAT
Tnf ACCACCATCAAGGACTCA AGGTCTGAAGGTAGGAAG
Il1b ACGGATTCCATGGTGAAGTC GAGTGTGGATCCCAAGCAAT
Il6 AGTCACAGAAGGAGTGGCTA CTGACCACAGTGAGGAATGT
Gpr41/Ffar3 GAAAAGAGTGGACCGCAGGA AGGGCAATCTGAGTGACAGC
Gpr43/Ffar2 TTCTTACTGGGCTCCCTGCC TACCAGCGGAAGTTGGATGC
Gpr109/Hcar CTGCTCAGGCAGGATCATCTGGAG CCCTCTTGATCTTGGCATGT