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. 2021 Jan 13;33(4):961–979. doi: 10.1093/plcell/koaa027

Figure 5.

Figure 5

Mutations in atxr5/6 lead to an increase in H3K27ac and H3K36ac in heterochromatin. (A) Normalized average distribution of H3K27ac and H3K36ac over protein-coding genes for Col, atxr5/6, gcn5, and atxr5/6 gcn5 in reference-adjusted reads per million (RRPM). TSS, transcription start site; TES, transcription end site. (B) Normalized average distribution and heatmap of H3K27ac and H3K36ac normalized reads surrounding the 323 H3K27ac/H3K36ac-enriched heterochromatic regions identified in atxr5/6 compared to Col. The regions are sorted based on levels (RRPM) of H3K27ac/H3K36ac enrichment. (C) Genome browser snapshot showing normalized H3K27ac and H3K36ac ChIP-seq data over a region of chromosome 5 that includes TE genes At5g29591 and At5g29602. The y-axis unit is RRPM. (D) Heatmap showing the RNA-seq reads mapping to the region ±3 kb around the center of the 323 H3K27ac/H3K36ac peaks as measured by RPKM in Col, atxr5/6, gcn5, and atxr5/6 gcn5. The regions are sorted based on expression level (RPKM).