Table 1.
Serine‐resistant mutants identified from the genome‐wide screen.
| Strain a | Genetic context | Serine resistance | Determinant for serine resistance |
|---|---|---|---|
| ΔybeC::erm | ybeC>, <glpQ < glpT | Yes | Deletion of ybeC |
| ΔybeC::lox72 | Yes | ||
| ΔglpQ::erm | ybeC>, <glpQ < glpT | Yes | Inhibition of ybeC expression |
| ΔglpQ::lox72 | No | ||
| ΔyloU::erm | yloU > yloV>, sdaAB > sdaAA > recG> | Yes | Overexpression of sdaAB‐AA |
| ΔyloU::lox72 | No | ||
| ΔyloV::erm | yloU > yloV>, sdaAB > sdaAA > recG> | Yes | Overexpression of sdaAB‐AA |
| ΔyloV::lox72 | No | ||
| ΔthrR::erm | spo0B > obg > thrR > pheA> | Yes | Deletion of thrR |
| ΔthrR::lox72 | Yes | Overexpression of hom‐thrCB | |
| ΔyutH::erm | yutH>, hom > thrC > thrB> | Yes | Overexpression of hom‐thrCB |
| ΔyutH::lox72 | No |
a
lox72 indicates the scar resulting from looping out of erythromycin‐resistant cassette.
The genes highlighted in bold are directly responsible for the resistance to serine.