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. 2021 Jun 8;10(6):1434. doi: 10.3390/cells10061434

Figure 1.

Figure 1

Inhibition of CyPA binding to CD147 does not play a role in SARS-CoV-2 entry. CaLu3 cells were treated with 1 µg/mL MEM-M6/6 CD147 blocking Ab (α-CD147 Ab) or equivalent mouse IgG (as isotype control) or plain medium (as control, ctr) for one hour before, and during SARS-CoV-2 infection. Samples were collected 48 hpi. (A) N1 RNA levels were analyzed by ddPCR and their relative abundance in cells is shown. N1 RNA levels were normalized to total RNA. N1 levels in CD147-blocking antibody treated samples were normalized to the levels in the corresponding IgG isotype-treated control. Mean ± s.e.m out of three biological replicates is shown. n.s. not significative, Student’s t test. (B,C) Lysates were analyzed by WB. (B) One experiment is shown as representative of three. Immunolabelling for CD147 showed that Ab treatment did not affect the levels of expression of the receptor either in the absence or in the presence of SARS-CoV-2. (C) Spike protein levels were normalized to GAPDH (loading control). Spike levels in CD147-blocking antibody treated samples were normalized to the levels in the corresponding IgG isotype-treated control. Mean ± s.e.m. out of three biological replicates is shown. n.s. not significant, Student’s t-test.