Figure 2.

Protease activity (conc) and inhibition assay (PMSF, EDTA) of Neoparamoeba perurans exoproteome samples from the attenuated and virulent cultures using azocasein (non-specific protease substrate) as a substrate. Trypsin (2 mg/mL) was used as positive control and sterile seawater as a negative. Results are expressed as a percent of the positive control. All datasets are represented as ±SEM from biological replicates. Two-way ANOVA followed by a Tukey post hoc test for multiple comparisons: Attenuated vs. phenylmethylsulfonyl fluoride (PMSF) and ethylenediaminetetraacetic acid (EDTA), **** (p < 0.0001); Virulent vs PMSF, * (p < 0.0284), Attenuated vs. Virulent, #### (p < 0.0001).