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. 2021 May 29;14(6):522. doi: 10.3390/ph14060522

Figure 1.

Figure 1

Effects of LPS and AUY-922 on pERK1/2, pP38, pJAK2 and pSTAT3 expression in mouse lungs. Western blot analysis of phosphorylated ERK1/2 (pERK1/2) and ERK1/2 (A), phosphorylated P38 (pP38) and P38 (B), phosphorylated JAK2 (pJAK2) and JAK2 (C), phosphorylated STAT3 (pSTAT3) and STAT3 (D) expression in lungs retrieved from mice 72 h after intratracheal injection of either vehicle (saline) or LPS (1.6 mg/kg) and post-treated (24 h after LPS) with an intraperitoneal injection of either AUY-922 (10 mg/kg each, dissolved in 10% DMSO) or vehicle (10% DMSO in saline). The signal intensity of protein bands was analyzed by densitometry. Protein levels of pERK1/2, pP38, pJAK2 and pSTAT3 were normalized to ERK1/2, P38, JAK2 and STAT3, respectively. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. vehicle (VEH) and $ p < 0.05, $$ p < 0.01, $$$ p < 0.001 vs. LPS, n = 3 animals per group. Means ± SEM.—indicates the absence, and + indicates the presence of the corresponding reagent in the treatments.