Table 4.
Inhibitory effects of C. aethiopica leaf total methanol extract and its fractions on superoxide anion generation and elastase release in FMLF/CB-induced human neutrophils.
| Sample | Superoxide Anion | Elastase Release | ||
|---|---|---|---|---|
| IC50 (μg/mL) a | Inhibition% | IC50 (μg/mL) a | Inhibition% | |
| CAL | >10 | 36.45 ± 2.58 *** | >10 | 15.27 ± 2.13 ** |
| CAL-A | 5.32 ± 0.46 | 68.68 ± 3.87 *** | 5.80 ± 0.34 | 65.18 ± 6.81 *** |
| CAL-B | 7.04 ± 1.28 | 58.31 ± 3.76 *** | 5.73 ± 0.64 | 68.08 ± 7.25 *** |
| CAL-C | >10 | 43.84 ± 1.94 *** | >10 | 24.51 ± 4.05 ** |
| CAL-D | >10 | 22.47 ± 5.53 * | b | b |
| CAL-E | >10 | 26.49 ± 2.73 *** | >10 | 5.30 ± 2.67 |
CAL: C. aethiopica leaf total methanol extract; CAL-A: C. aethiopica n-hexane fraction; CAL-B: C aethiopica dichloromethane fraction; CAL-C: C. aethiopica ethyl acetate fraction; CAL-D: C. aethiopica n-butanol fraction; CAL-E: C. aethiopica remaining aqueous fraction. a Percentage of inhibition at 10 μg/mL concentration. Results are presented as mean ± S.E.M. (n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the control value (fMLF/CB). b Sample CAL-D had promoting effects (23.86 ± 3.78% in the presence of CB) on elastase release in human neutrophils.