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. 2021 May 27;14(6):518. doi: 10.3390/ph14060518

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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UV-vis absorption spectra recorded for (a,d) complex 1, (b,e) complex 3 and (c,f) complex 4 in the presence of various equivalents of HSA or mim, respectively at pH = 7.4. The numbers indicate the range of the c_HSA/c_complex or c_mim/c_complex ratios. In case of PTA complexes 24 h incubation time was used. {T = 25.0 °C, pH = 7.40 PBS’ buffer, c_complex = 210 μM, c_HSA= 6.3–71.4 μM or c_mim = 29–210 μM (a,b,d,e); c_complex = 116 μM, c_HSA= 6.3–71.4 μM or c_mim = 29–1160 μM with 8% (v/v) DMSO (c,f); l = 1 cm}.