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Conditional binding constants of the compounds at binding sites I and II of HSA determined by spectrofluorometric Trp-214 quenching (logKQ’) and site marker (WF or DG) displacement (logKWF’ and logKDG’) measurements. {pH = 7.40 PBS’ buffer; T = 25 °C}.
| logKQ’ | logKWF’ | logKDG’ | ||
|---|---|---|---|---|
| 1 | 5.81 ± 0.03 | (titration) | 6.16 ± 0.03 | 5.80 ± 0.03 |
| 2 | <4 | (titration) | – | – |
| 3 | 6.18 ± 0.03 | (titration) | 5.98 ± 0.03 | 5.61 ± 0.03 |
| 4 | 4.46 ± 0.03 5.39 ± 0.03 |
(titration) (48 h) | – | – |
