Table 2.
Effect of liposomes composition on delivery of PIP-2 to lungs. PIP-2 in liposomes was delivered to mice by a bolus IV infusion. After 2 h lungs were cleared of blood and PLA2 activity of the lung homogenate was measured. % inhibition = value relative to no PIP-2. * % of total lipid; all liposomes also contained 15% cholesterol. Ϯ Mean ± SD for n = 3 or ± range for n = 2.
| Composition * DPPC: PC: PG |
PIP-2 | n | Activity ϯ nmol/mg prot/hr |
Inhibition % |
|---|---|---|---|---|
| 50/25/10 | NO | 2 | 8.83 ± 0.10 | ----- |
| 50/25/10 | YES | 3 | 1.64 ± 0.07 | 81.4 |
| 0/75/10 | YES | 3 | 1.72 ± 0.10 | 80.5 |
| 75/0/10 | YES | 2 | 1.84 ± 0.02 | 79.2 |
| 55/30/10 | YES | 2 | 2.51 ± 0.12 | 71.7 |