Table 3.
Effect of intratracheal (IT) PIP-2 on manifestations of VILI. Male mice were ventilated for 6hr with tidal volume of 4 (low) or 12 (high, VILI) mL/Kg, respiratory rate 120/min, PEEP 2 cm H2O. Mice were administered PIP-2 (2 or 10 µg/g body weight) encapsulated in liposomes by IT bolus infusion. After 6 h, mice were sacrificed, and lungs processed as described in Materials and Methods. Results are mean ± SD for n = 4. * p < 0.05 vs. all other groups. ϯ p < 0.05 vs. Control, Low tidal volume (±PIP-2) and VILI. There are no significant differences between 2 and 10 ug/g PIP-2.
| Condition | # of cells in BALf (×104/g body wt) |
Total Protein in BALf (mg/g Body wt |
Wet/Dry Weight Ratio of Lung |
TBARS pmol/mg prot. |
8—Isoprotanes pmol/mg prot |
Protein Carbonyls nmol/mg prot |
|---|---|---|---|---|---|---|
| Control (non-ventilated) |
0.97 ± 0.12 | 76.9 ± 4.2 | 5.61 ± 0.04 | 74.6 ± 4.6 | 33.1 ± 6.0 | 5.63 ± 0.48 |
| Low tidal volume | 1.03 ± 0.06 | 80.1 ± 4.4 | 6.02 ± 0.26 | 81.7 ± 3.0 | 37.8 ± 4.2 | 5.90 ± 0.46 |
| Low tidal volume + PIP-2 | 1.01 ± 0.08 | 78.8 ± 3.6 | 5.98 ± 0.22 | 79.9 ± 4.2 | 36.2 ± 2.4 | 5.82 ± 0.48 |
| High tidal volume (VILI) * | 19.6 ± 2.84 | 235 ± 20 | 10.8 ± 0.42 | 530 ± 14.4 | 174 ± 9.8 | 22.3 ± 1.56 |
| VILI + PIP-2 (2 µg/g) ϯ |
8.4 ± 0.62 | 102 ± 16.2 | 8.14 ± 0.22 | 206 ± 24 | 75.9 ± 2.0 | 8.85 ± 0.84 |
| VILI + PIP-2 (10 µg/g) ϯ |
8.1 ± 0.46 | 107 ± 16.2 | 7.62 ± 0.36 | 202 ± 12.4 | 76.5 ± 2.4 | 8.12 ± 0.34 |