Table 4.
Effect of intravenous (IV) PIP-2 on manifestations of VILI. Male mice were ventilated for 6 h with tidal volume of 12 mL/Kg, respiratory rate 120/min, PEEP 2 cm H2O. Control mice breathed normally (no mechanical ventilation). Mice were administered PIP-2 (2 µg/g body weight) encapsulated in liposomes by IV bolus injection. Mice were sacrificed after 6h, and lungs were cleared of blood, lavaged, and processed as described in Materials and Methods. Results are mean ± SD for n = 4. * p < 0.05 vs. Control and vs. VILI+ PIP-2; ϯ p < 0.05 vs. Control. Statistics represent all values in the indicated row.
| Condition | # of Cells in BALf (×104/g body wt) |
Total Protein in BALf (mg/g Body wt |
Wet/Dry Weight Ratio of Lung | TBARS pmol/mg prot. |
8—Isoprostanes pmol/mg prot |
Protein Carbonyls nmol/mg prot |
|---|---|---|---|---|---|---|
| Control (non-ventilated) |
1.04 ± 0.14 | 82.4 ± 10.0 | 5.94 ± 0.50 | 80.5 ± 3.2 | 31.5 ± 5.4 | 5.48 ± 0.58 |
| VILI * | 18.9 ± 1.16 | 218 ± 19 | 10.4 ± 0.70 | 512 ± 26 | 178 ± 34 | 21.1 ± 1.32 |
| VILI + PIP-2 ϯ | 8.17 ± 0.28 | 103 ± 5.6 | 7.4 ± 0.24 | 186 ± 36 | 81.3 ± 7.2 | 8.44 ± 1.38 |