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. 2021 May 30;10(6):879. doi: 10.3390/antiox10060879

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of fisetin on proliferation and migration of SaOs-2 cells. The distance between edges was monitored under inverted microscope to determine gap closure upon fisetin treatment (A). Representative pictures of cells at the bottom side of the inserted chamber after 48 h incubation stained with Hoechst-33342 (B). The number of migrated cells was counted and reported as mean ± SD; n = 3 (C). YAP expression in SaOs-2 cells upon fisetin treatment for 48 h (D). Alkaline phosphatase activity staining (E). The data presented in subfigure (C,D) were analyzed by Mann–Whitney U test. Bars indicate mean values ± standard deviation (SD) and asterisks indicate significant differences, *** p < 0.001; n.s. = no significant difference.