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. 2021 Jun 9;13(12):2889. doi: 10.3390/cancers13122889

Figure 1.

Figure 1

PAM selectively affects mesenchymally-shifted cancer cells. Breast cancer cell lines (MDA-MB-231/MDA-MB-468/MCF-7; pink) and EMT/MET cell line systems in breast (PMC42-ET/LA; grey) and bladder (5673/TSU-Pr1/B1/B2; blue) cancer were starved with serum-free medium for 3 h and treated with PAM in 0%, 20%, 50%, 70%, and 100% 10PAM for 12 h (a) or 70% 10PAM for 0, 3, 6, 9, 12, and 24 h (b). Cell assay viability as per live/dead cell assay (mean ± SEM of 0% or 0 h PAM, respectively) was calculated by IN Carta analysis. The dose response for 12 h treatment (c) and time course (d) of 70% 10PAM treatment of breast cancer cell lines (MDA-MB-231/MDA-MB-468/MCF-7; pink), replotted from (a,b), respectively; the dose response for 12 h treatment (e) and time course of 70% 10PAM treatment (f) of EMT/MET the PMC42 cell line system (ET/LA; grey); and the dose response for 12 h treatment (g) and time course of 70%10PAM treatment (h) of bladder cancer cells (5673/TSU-Pr1/B1/B2; blue) cells are shown. The mean and SEM of the average for each data point from each of the 3 experiments are shown. “*” represents p < 0.05, “**” represents p < 0.01, “***” represents p < 0.001, and “****” represents p < 0.0001.