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. 2021 Jun 4;13(6):1070. doi: 10.3390/v13061070

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree of the Hemagglutinin Esterase (HE) gene of Bovine coronavirus (BCoV). Local isolates. (A) Local strains’ (labeled in red) alignment with selected reference BCoV HE sequences. Phylogenetic tree was generated based on full HE gene sequences. Phylogenetic trees were generated via the neighbor-joining method with bootstrap analysis (1000 replicates, >70%). The scale bar shows the number of substitutions per site. We used HCoV OC43 as an outgroup strain. HE sequences retrieved from GenBank and embedded in the figure for each strain. (B) Deduced amino acid sequences of local isolates aligned to reference strain Mebus. Amino acid alignment showing only disagreements residues compared to reference strain Mebus.