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. 2021 Jun 3;11(6):502. doi: 10.3390/jpm11060502

Figure 5.

Figure 5

Sarcoplasmic reticulum Ca-load and NCX activity. SR Ca load and NCX function were assessed under caffeine (20 mM/L) application. Caffeine transients were measured in LV and RV cardiomyocytes loaded with the Fura-2 AM fluorescence indicator (2 µM). (A,B) Amplitude of caffeine-induced Ca transients showing SR Ca load in LV and RV myocytes, as indicated by the fura-2 ratio (340:380 nm). (C,D) T50: the time constant for 50% decay of the caffeine-induced Ca transient. (E,F) Tau of caffeine-induced Ca transient decay. n = 27 LV and 28 RV cells for WT (N = 3 hearts); n = 27 LV and 29 RV cells for R14del-PLN (N = 4 hearts). Data are expressed as mean ± SEM for the number of cells, and statistical analyses were performed by Student’s unpaired t-test. ** p ≤ 0.001 vs. WT-PLN.