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. 2021 Jun 3;11(6):502. doi: 10.3390/jpm11060502

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Assessment of PLN levels in LV and RV. (A) Representative immunoblots of PLN and SERCA2a protein levels in LV from WT-PLN and R14del-PLN. (B) Quantitative assessment of PLN protein levels in LV. (C) Quantitative assessment of SERCA2a protein levels in LV. Calsequestrin (CSQ) was used as a loading control. N = 6 LV samples from WT and N = 6 LV samples from R14del-PLN mice. (D) Representative immunoblots of PLN and SERCA2a protein levels in RV from WT-PLN and R14del-PLN. (E) Quantitative assessment of PLN protein levels in RV. (F) Quantitative assessment of SERCA2a protein levels in RV. Calsequestrin (CSQ) was used as a loading control. N = 6 RV samples from WT and N = 6 RV samples from R14del-PLN mice. (G,H) The PLN/SERCA2a ratio in RV and LV samples. Twelve- to fourteen-week-old male mice were used. Data are expressed as mean ± SEM for the number of hearts and statistical analyses were performed by two-tailed unpaired t-test. A p-value of <0.05 was considered statistically significant.