Figure 8.

Assessment of SR calcium cycling protein levels in RV and LV of WT and R14del-PLN mice. (A) Representative immunoblots of Ca cycling protein levels in WT and R14del ventricles. (B,C) Quantitative assessment of SERCA2a protein levels. (D,E) Quantitative assessment of HRC protein levels. (F,G) Quantitative assessment of NCX protein levels. (H,I) Quantitative assessment of HAX-1 protein levels. Calsequestrin (CSQ) was used as a loading control. N = 4 RV and LV samples from WT and N = 4 RV and LV samples from R14del-PLN mice. Twelve- to fourteen-week-old male mice were used. Data are expressed as mean ± SEM for the number of hearts and statistical analyses were performed by two-tailed unpaired t-test. A p-value of <0.05 was considered statistically significant.