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. 2021 May 30;19(6):318. doi: 10.3390/md19060318

Figure 2.

Figure 2

Effects of ECE and DK on the NLRP3 inflammasome-induced pyroptosis in the liver of the HFD-induced NAFLD mouse model. In liver tissue, (A–C) the ASC (upper row of A and B) and NLRP3 (lower row of A and C) expression levels were increased by HFD/saline and decreased by ECE or DK treatment. Scale bar, 200 µm. (D and E) Immunoblotting results show expression of Casp1, cleaved-Casp1, GSDMD, and cleaved-GSDMD. (F and G) The PI-positive cell numbers in the liver were increased by HFD/saline and decreased after treatment with ECE or DK. Data are mean ± SD. p < 0.05; a–f; Same letters indicate nonsignificant differences between groups as determined by multiple comparison (Mann–Whitney U test). ASC, apoptosis-associated speck-like protein containing a CARD; Casp1, caspase-1; DK, dieckol; ECE, Ecklonia cava extract; GSDMD, gasdermin D; HFD, high-fat diet; NFD, normal fat diet; NLRP3, NOD-like receptor family, pyrin domain containing 3; PI, propidium iodide.