Table 1.
Primers used to amplify gene segments of A(H1N1)pdm09 viruses.
| Gene | Primer | Binding Position | Sequence * |
|---|---|---|---|
| HA | Forward 1 | 1–22 | TGT AAA ACG ACG GCC AGT ATGAAGGCAATACTAGTAGTTCTG |
| HA | Reverse 1 | 901–921 | CAG GAA ACA GCT ATG ACC GAGGCTGGTGTTTATAGCACC |
| HA | Forward 2 | 802–822 | TGT AAA ACG ACG GCC AGT CCGAGATATGCATTCGCAATG |
| HA | Reverse 2 | 1676–end | CAG GAA ACA GCT ATG ACC TTAAATACATATTCTACACTGTAGAG |
| NA | Forward 1 | 1–26 | TGT AAA ACG ACG GCC AGT ATGAATCCAAACCAAAAGATAATAAC |
| NA | Reverse 1 | 823–842 | CAG GAA ACA GCT ATG ACC CAGGAGCATTCCTCATAGTG |
| NA | Forward 2 | 706–729 | TGT AAA ACG ACG GCC AGT GGTTCTTGCTTTACTGTAATGACC |
| NA | Reverse 2 | 1386–1410 | CAG GAA ACA GCT ATG ACC TTACTTGTCAATGGTAAATGGCAAC |
| NA ● | Forward | 727–748 | CTGTAATGACCGATGGACCAAG |
| NA ● | Reverse | 913–935 | CAGATTCTGGTTGAAAGACACCC |
| PB1 | Forward 1 | 1–22 | TGT AAA ACG ACG GCC AGT ATGGATGTCAATCCGACTCTAC |
| PB1 | Reverse 1 | 996–1017 | CAG GAA ACA GCT ATG ACC CATGCTCAGGATGTTTCTGAAC |
| PB1 | Forward 2 | 597–618 | TGT AAA ACG ACG GCC AGT GGTCACGCAAAGAACAATAGG |
| PB1 | Reverse 2 | 1521–1542 | CAG GAA ACA GCT ATG ACC CACTCCAAAGCTGGGTAGCT |
| PB1 | Forward 3 | 1301–1321 | TGT AAA ACG ACG GCC AGT CAATATACTGGTGGGATGGGC |
| PB1 | Reverse 3 | 2251–end | CAG GAA ACA GCT ATG ACC TTATTTTTGCCGTCTGAGTTCTTC |
| PB2 | Forward 1 | 1–23 | TGT AAA ACG ACG GCC AGT ATGGAGAGAATAAAAGAACTGAGAG |
| PB2 | Reverse 1 | 998–021 | CAG GAA ACA GCT ATG ACC CTTTCTTGACTGATGATCCGC |
| PB2 | Forward 2 | 603–626 | TGT AAA ACG ACG GCC AGT GGTGGCGTACATGCTAGAAAG |
| PB2 | Reverse 2 | 1552–1573 | CAG GAA ACA GCT ATG ACC CAGTTCCTTGCGTTTCACTGAC |
| PB2 | Forward 3 | 1230–1248 | TGT AAA ACG ACG GCC AGT GATCAAGGCAGTTAGGGGC |
| PB2 | Reverse 3 | 2258–end | CAG GAA ACA GCT ATG ACC CTAATTGATGGCCATCCGAATTC |
| PA | Forward 1 | 1–23 | TGT AAA ACG ACG GCC AGT ATGGAAGACTTTGTGCGACAATG |
| PA | Reverse 1 | 996–1017 | CAG GAA ACA GCT ATG ACC CTTCCAAGCCATGAGGTAATTG |
| PA | Forward 2 | 616–36 | TGT AAA ACG ACG GCC AGT GAGATTACAGGAACTATGCGC |
| PA | Reverse 2 | 1519–1540 | CAG GAA ACA GCT ATG ACC CATTTCTCAAATGAGACCTTCC |
| PA | Forward 3 | 1162–1184 | TGT AAA ACG ACG GCC AGT GGAGACCTTAAACAGTATGACAG |
| PA | Reverse 3 | 2130–end | CAG GAA ACA GCT ATG ACC CTACTTCAGTGCATGTGTGAGG |
| M | Forward | 1–21 | TGT AAA ACG ACG GCC AGT ATGAGTCTTCTAACCGAGGTC |
| M | Reverse | 959–982 | CAG GAA ACA GCT ATG ACC TTACTCTAGCTCTATGTTGACAAA |
| NP | Forward 1 | 1–20 | TGT AAA ACG ACG GCC AGT ATGGCGTCTCAAGGCACC |
| NP | Reverse 1 | 801–821 | CAG GAA ACA GCT ATG ACC GATTTATGTGCAACTGATCCC |
| NP | Forward 2 | 702–721 | TGT AAA ACG ACG GCC AGT CCAGAGGGCAATGATGGATC |
| NP | Reverse 2 | 1477–end | CAG GAA ACA GCT ATG ACC TCAACTGTCATACTCCTCTGC |
| NS | Forward | 1–20 | TGT AAA ACG ACG GCC AGT ATGGACTCCAACACCATGTC |
| NS | Reverse | 840–863 | CAG GAA ACA GCT ATG ACC GTAGAAACAAGGGTGTTTTTTATC |
* The sequence of the M13 tail is indicated in blue. ● Primers used for NA sequencing.