. 2021 Jun 1;9(6):1196. doi: 10.3390/microorganisms9061196

Table 2.

Amino acid changes identified by sanger sequencing in selected A(H1N1)pdm09 variants.

Virus	Passage No.	Oseltamivir (μM)	IFN λ1 (ng/mL)	HA *	NA **	PA	PB1	PB2	NP	M
N09 Stock	-	-	-	-	D344N, D354G	-	-	-	-	-
UTR p3	3	-	-	-	D344N, D354G	ns	ns	ns	ns	ns
UTR p4	4	-	-	K62R, G239D, Q240R	D344N, D354G	ns	ns	ns	ns	ns
UTR p9	9	-	-	K62R, G239D, Q240R	D344N, D354G	V100L ^■	A1920T °	T303S ^■	-	K57R ^■
OS p7	7	640	-	ns	H275Y ^■, D344N, D354G	ns	ns	ns	ns	ns
OS p8	8	640	-	ns	H275Y ^■, D344N, D354G	ns	ns	ns	ns	ns
OS p9	9	640	-	-	H275Y, D344N, D354G	N65K ^■	-	-	-	K57R ^■
λ p9	9	-	960	K62R, G239D, Q240R	D344N, D354G	-	-	I57M ^■	P318Q ^■	K57R ^■
OS/λ p9	9	640	960	-	D344N, D354G	-	-	-	-	K57R ^■
OS/λ p10	10	640	960	ns	H275Y ^■, D344N, D354G	ns	ns	ns	ns	ns
OS/λ p11	11	640	960	ns	H275Y ^■, D344N, D354G	ns	ns	ns	ns	ns
OS/λ p12	12	640	960	ns	H275Y ^■, D344N, D354G	ns	ns	ns	ns	ns

* Based on H1 numbering as in [60]. ** Based on N1 numbering as in [61]. ^■ Present as a mixed population. ° Silent mutation, for which the nucleotide substitution is shown. ns = not sequenced.