Table 1.
Relative quantification of Leg1 and Leg2 in chickpea chymotryptic hydrolysates by ultrahigh performance-tandem mass spectrometry in scheduled multiple reaction monitoring mode (UHPLC–MS/MS-sMRM). The analytical parameters of the method are displayed. QILQWQ was used as internal standard. The net charge is given by superscript numbers (1+,2+,3+,4+).
| Peptide Sequence | Theoretical Mass (Da) | RT 1 (min) | Precursor Ion (m/z) | Quantifier Ion (m/z) | Collision Energy (eV) | Confirmation Ions (m/z) | |
|---|---|---|---|---|---|---|---|
| RIKTVTSFDLPALRFLKL (Leg1) | 2118.59 | 36.1 | 530.334+ | 957.021+ | 30.4 | 710.422+ | 581.342+ |
| RIKTVTSFDLPALRWLKL (Leg2) | 2157.62 | 36.7 | 540.084+ | 729.932+ | 27.0 | 686.412+ | 780.452+ |
| QILQWQ | 814.94 | 25.2 | 408.222+ | 242.163+ | 22.6 | 333.162+ | 355.231+ |
1 Retention time.