Table 2.
Stability of Leg1 during storage at 20 °C for 0–28 days. The residual antimicrobial activity against E. coli was measured by the resazurin assay and is expressed as minimal inhibitory concentration (MIC). For comparison, the stability of the variant Leg2 and the approved preservatives nisin, sodium benzoate, potassium sorbate, and sodium nitrite were analyzed in the same way. The same results were obtained during storage at 4 °C for 0–28 days.
| MIC (µM) at Day(s) | |||||||
|---|---|---|---|---|---|---|---|
| 0 | 1 | 3 | 7 | 14 | 21 | 28 | |
| Leg1 | 62.5 | 62.5 | 62.5 | 62.5 | 125 | 125 | 125 |
| Leg2 | 62.5 | 62.5 | 62.5 | 62.5 | 125 | 125 | 125 |
| Nisin | 125 | 125 | 125 | 125 | 125 | 125 | 125 |
| Sodium benzoate | 40,000 | 40,000 | 40,000 | 40,000 | 40,000 | 40,000 | 40,000 |
| Potassium sorbate | 20,000 | 20,000 | 20,000 | 20,000 | 20,000 | 20,000 | 20,000 |
| Sodium nitrite | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 | 10,000 |