Table 3.
Antimicrobial activity of Leg1 against E. coli and B. subtilis at different temperatures expressed as minimal inhibitory concentration (MIC). For comparison, the variant Leg2 and the approved preservatives nisin, sodium benzoate, potassium sorbate, and sodium nitrite were tested in the same way.
| MIC in µM (mg/mL) at Temperatures | ||||
|---|---|---|---|---|
| 37 °C | 20 °C | 37 °C | 20 °C | |
| E. coli | B. subtilis | |||
| Leg1 | 62.5 (0.13) | 15.6 (0.03) | 15.6 (0.03) | 7.8 (0.02) |
| Leg2 | 62.5 (0.13) | 15.6 (0.03) | 15.6 (0.03) | 4.0 (0.01) |
| Nisin | 125 (0.42) | 15.6 (0.05) | 7.81 (0.03) | 1.0 (0.004) |
| Sodium benzoate | 40,000 (5.8) | 20,000 (2.9) | 80,000 (11.5) | 10,000 (1.5) |
| Potassium sorbate | 20,000 (3.0) | 20,000 (3.0) | 160,000 (24.0) | 20,000 (3.0) |
| Sodium nitrite | 10,000 (0.68) | 20,000 (1.36) | 40,000 (2.76) | 20,000 (1.36) |