. 2021 May 31;10(6):884. doi: 10.3390/antiox10060884

Table 2.

Effects of the supplementation of the maternal diet without (Ctrl) or with HTX on the mean values of the concentration of neurotransmitters in the hippocampus of 100-day-old fetuses, and 1-month- and 6-month-old pigs.

		Fetuses	1 Month	6 Months	p-Values
		Fetuses	1 Month	6 Months	Treatment	Age	Interaction
NA	Ctrl	60.71 ± 3.28 ^Aa	91.21 ± 3.88 ^Ab	110.24 ± 3.98 ^Ab	0.096	<0.001	0.001
NA	HTX	81.25 ± 3.89 ^Ba	92.42 ± 4.64 ^Aab	106.90 ± 7.34 ^Ab	0.096	<0.001	0.001
DA	Ctrl	97.87 ± 9.25	18.55 ± 2.31	48.99 ± 3.78	0.643	<0.001	0.312
DA	HTX	88.40 ± 4.64	19.56 ± 1.88	42.76 ± 3.96	0.643	<0.001	0.312
DOPAC	Ctrl	54.37 ± 6.60 ^Aa	16.51 ± 2.66 ^Ab	28.57 ± 0.67 ^Aa	0.002	<0.001	0.023
DOPAC	HTX	79.54 ± 8.64 ^Ba	19.95 ± 2.32 ^Ab	31.61 ± 1.60 ^Ac	0.002	<0.001	0.023
HVA	Ctrl	209.07 ± 13.43 ^Aa	35.91 ± 1.97 ^Ab	40.94 ± 1.11 ^Ab	0.024	<0.001	0.015
HVA	HTX	275.01 ± 13.60 ^Ba	36.12 ± 2.33 ^Ab	46.74 ± 3.01 ^Ac	0.024	<0.001	0.015
DOPAC/DA	Ctrl	0.79 ± 0.10	2.04 ± 0.91	0.66 ± 0.04	0.037	0.001	0.066
DOPAC/DA	HTX	1.04 ± 0.15	1.10 ± 0.10	0.80 ± 0.06	0.037	0.001	0.066
HVA/DA	Ctrl	2.60 ± 0.18 ^Aa	4.50 ± 1.77 ^Aa	0.95 ± 0.06 ^Ab	0.208	<0.001	0.004
HVA/DA	HTX	3.22 ± 0.13 ^Ba	2.08 ± 0.13 ^Ab	1.20 ± 0.10 ^Ac	0.208	<0.001	0.004
(DOPAC + HVA)/DA	Ctrl	3.40 ± 0.26 ^Aa	6.54 ± 2.68 ^Aa	1.61 ± 0.10 ^Ab	0.100	<0.001	0.011
(DOPAC + HVA)/DA	HTX	4.26 ± 0.22 ^Ba	3.18 ± 0.18 ^Ab	2.00 ± 0.15 ^Ac	0.100	<0.001	0.011
DOP total	Ctrl	361.31 ± 22.42	70.97 ± 4.77	118.50 ± 4.00	0.063	<0.001	0.061
DOP total	HTX	442.95 ± 20.04	75.64 ± 5.34	121.11 ± 6.22	0.063	<0.001	0.061
5-HT	Ctrl	177.71 ± 7.96	264.34 ± 16.57	231.35 ± 11.55	0.203	<0.001	0.216
5-HT	HTX	213.97 ± 10.95	274.56 ± 16.94	235.98 ± 21.42	0.203	<0.001	0.216
5-HIAA	Ctrl	88.67 ± 4.15	94.39 ± 5.23	93.66 ± 3.61	0.414	0.475	0.547
5-HIAA	HTX	94.24 ± 3.06	94.21 ± 5.11	96.70 ± 4.84	0.414	0.475	0.547
5-HIAA/5-HT	Ctrl	0.52 ± 0.02	0.37 ± 0.02	0.42 ± 0.02	0.502	<0.001	0.410
5-HIAA/5-HT	HTX	0.47 ± 0.02	0.36 ± 0.02	0.45 ± 0.03	0.502	<0.001	0.410
IND total	Ctrl	266.38 ± 11.14	358.73 ± 20.38	325.01 ± 13.17	0.209	<0.001	0.278
IND total	HTX	308.21 ± 13.05	368.77 ± 19.72	332.68 ± 23.46	0.209	<0.001	0.278

Concentrations are presented as the mean ± SE. Units ng/g tissue. Different capital letters (Aa = Ab = Ac = Aab) ≠ (Ba = Bb = Bab) represent significant differences between treatments for each age (p < 0.05). Different lowercase letters (Aa = Ba = Aab = Bab) ≠ (Ab = Bb = Aab = Bab) ≠ Ac represent significant differences between ages for the same treatment (p < 0.05). NA: noradrenalin; DA: dopamine; DOPAC: 3,4-dihydroxyphenyl acetic acid; HVA: homovanillic acid; 5-HT: serotonin/5-hydroxytryptamine; 5-HIAA: 5-hydroxyindoleacetic acid; DOP total: total dopaminergic neurotransmitters; IND total: total serotoninergic neurotransmitters.