Figure 4.

TNFα induces massive Dil over [³H]CE lipid accumulation from LDL. pHAECs in serum medium were pre-treated with the indicated concentration of human TNFα for 24 h. Subsequently, the cells were treated in the continued presence of the previous TNFα concentration and 50 µg/mL [³H]CE-LDL (A) or 5 µg/mL Dil-LDL (B) without serum for 24 h. A, n = 4, B, n = 3. The cells were treated without (Ctrl), (C), or with 100 ng/mL TNFα (D), as above, in the presence of increasing concentration of Dil-LDL. n = 5. *, **, ***, p < 0.05, 0.01, 0.001, relative to 0 ng/mL TNFα or 1 µg/mL Dil-LDL. #, ##, p < 0.05, 0.01, vs. previous TNFα dose. $$, $$$, p < 0.01, 0.001, relative to the corresponding Dil-LDL concentration under Ctrl condition.