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TNFα upregulates cell surface LDLR protein. (A–F), Confluent pHAECs in serum medium were pre-treated with 0 or 100 ng/mL TNFα (+) for 24 h in serum medium. The cells were then treated and lysed for total (Total Lysates) and biotinylated cell surface (Cell Surface) proteins, as explained under Materials and Methods, Section 4.14. ABCA1 bands (A,B) at ~220 and 110 kD are indicated. n = 3. *, **, ***, p < 0.05, 0.01, 0.001, vs. corresponding Ctrl.
