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. 2021 Jun 9;22(12):6236. doi: 10.3390/ijms22126236

Figure 9.

Figure 9

TNFα promotes rapid association of LDLR with it antibody. pHAECs in serum medium were pre-treated with 0 (Ctrl) or 100 ng/mL TNFα (TNFα) for 24 h. Subsequently, in the continued presence of TNFα, the media were replaced with 5 µg/mL control normal goat IgG or LDLR antibody (LDLR Ab) for 0, 5, 30, or 120 min at 37 °C. The surface-accessible LDLR was then determined as detailed under Experimental Procedures. (A) 20× objective magnification of LDLR detected after 30 min. (B) 40× confocal detection of LDLR in TNFα-treated pHAECs at 0 and 5 min. The arrows show the membrane distribution of the LDLR in focus, while the arrowhead shows internalized LDLR. (C) Surface-accessible LDLR as a function of time under control condition. (D) As C, in the presence of TNFα. n = 4. **, p < 0.01 relative to control at the same time.