Figure 10.

Antioxidant suppresses TNFα-induced Dil-LDL accumulation. (A) pHAECs were pre-treated with 0.05% DMSO (Vehicle) or TNFα-cascade inhihitors, as indicated: NF-kBi (25 µM). Two different forms were tested. ASK1i (5 µM), p38i (1 µM), JNKi (5 µM), or pan-caspase inhibitor, Caspasei (5 µM) for 30 min in serum-free medium. Subsequently, serum-free 3 µg/mL Dil-LDL, with 100 ng/mL TNFα, as indicated, was added, followed by 24 h incubation. n = 3. (B) Cells were treated with 1% DMSO vehicle with 100 ng/mL TNFα as indicated, in the presence of 0, 1, 5, or 20 mM TMTU and 3 µg/mL Dil-LDL. Subsequently, the cells were cultured for 24 h. n = 4. ***, ###, p < 0.001 vs. 0 µg/mL TNFα and 0 mM TMTU in the presence of TNFα, respectively.