Figure 11.

TNFα does not affect AP to BL release of degraded LDL protein. (A) Diagram of the experimental setup in (B,C) in which pHAECs were grown to confluence on 3 µm (pore size) polycarbonate filters, with ¹²⁵I-LDL in the AP medium. (B) pHAECs in serum medium were pre-treated with 0 (Ctrl) or 5 ng/mL TNFα on the BL side for 48 h as described under Materials and Methods, Transwell Insert Experiments. Under serum-free conditions, 10 µg/mL ¹²⁵I-LDL was added to the AP side (No Competitor) or with 30 folds unlabeled LDL, (+) 30× LDL, in the continued presence of TNFα. After 3 h, the BL media were collected, and non-intact ¹²⁵I-LDL was measured as described under Experimental Procedures. (C) The experiment was performed as in B, followed by detection of intact BL ¹²⁵I-LDL. n = 3. *,**, p < 0.05, 0.01, respectively.