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. 2021 Jun 9;9(6):1253. doi: 10.3390/microorganisms9061253

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Neighbour-joining phylogenetic trees based on (A) BLAST analysis of 16S rRNA gene sequences obtained by PCR amplification of DNA extracted from six sub-Antarctic strains and (B) alkane 1-monooxygenase gene (alkB) gene sequences from whole genome sequences of Williamsia spp. 1135 and 1138 and Rhodococcus spp. 1139, 1159, 1163 and 1168. Bootstrap values shown at branch nodes were based on 1000 re-samplings and the alkB gene sequences in (B) are from published genomes of the type strains for the species selected. The sub-Antarctic strains are marked with a red arrow in panel (A).