Table 2.
Quality and quantity scores for gDNA obtained from large-scale (50 mL) cultures of six sub-Antarctic strains.
| Strain Name | ng/µL a | GQN a | Fragment Size Range (Kbp) a | Average Size (Kbp) a |
A 260/280 b |
A 260/230 b |
Q30 (%) c | N50 d (Kbp) |
L50 d | Contigs d | GC Content (%) d |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Williamsia sp. 1135 | 66.1 | 8.1 | 13.28–44.50 | 28.721 | 1.92 | 1.84 | 64.36 | 142.77 | 14 | 109 | 64.7 |
| Williamsia sp. 1138 | 319 | 9.8 | 57.24–62.78 | 59.424 | 1.93 | 1.93 | 63.92 | 191.09 | 12 | 54 | 64.8 |
| R. qingshengii 1139 | 169.7 | 9.3 | 41.57–56.44 | 48.133 | 1.94 | 1.75 | 62.70 | 94.97 | 24 | 192 | 62.3 |
| R. erythropolis 1159 | 106.2 | 8.7 | 11.63–48.44 | 27.142 | 1.78 | 1.78 | 65.11 | 196.55 | 13 | 114 | 62.3 |
| Rhodococcus sp. 1163 | 40.5 | 8.7 | 15.73–47.69 | 32.799 | 1.99 | 1.87 | 64.67 | 329.55 | 5 | 43 | 62.3 |
| Rhodococcus sp. 1168 | 66.3 | 9.5 | 9.72–51.78 | 27.334 | 2.01 | 1.88 | 66.25 | 154.59 | 10 | 97 | 62.1 |
a DNA concentration and GQN obtained from Fragment Analyser data. b A260/A280 and A260/A230 ratio were obtained by Nano Drop spectrophotometry. c Obtained from Macrogen: Q30 is the % of reads that have a phred quality score of over 30. d Obtained after assembly using ABySS software; N50 is the length of the shortest contig that provides 50% of the genome when information in contigs above this size is summed [1]; DNA GC content of assembled sequences.