Figure 4.

Effects of CTBS treatment on NF-κB expression in EAE rats. Triple immunofluorescence labeling directed to astrocyte marker GFAP (blue), microglial marker IBA-1 (green), and NF-κB (red). Faint colocalization of NF-κB-ir and GFAP was observed in control sections (A). In EAE sections, a marked increase in NF-κB-ir was observed predominantly colocalizing with GFAP⁺ cells (B). CTBS treatment decreased immunostaining of NF-κB, and only scattered NF-κB⁺/GFAP⁺ cells were observed (C). Scale bar corresponds to 50 μm.