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Effects of CTBS treatment on IL-10 expression in EAE rats. Double immunofluorescence labeling directed to astrocyte marker GFAP (blue) and anti-inflammatory cytokine IL-10 (red). Control sections revealed modest colocalization of IL-10 and GFAP (A), which was barely detectable in EAE animas (B). CTBS treatment led to marked increase in immunostaining of IL-10, which was confined to quiescent GFAP+ cells (C). Scale bar corresponds to 50 μm.
