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. 2021 Jun 1;11(6):736. doi: 10.3390/brainsci11060736

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of CTBS treatment on AMP-based enzyme histochemistry in lumbar spinal cords of EAE rats. Enzyme histochemistry in the presence of AMP as a substrate labeling structures that exhibit eN/CD73 activity in the spinal cord of control, EAE, and CTBS-treated EAE sections. Control sections (A) exhibited diffuse staining patterns localized mainly in gray matter (B), while white matter was devoid of staining. (C) EAE sections reveled (D) a marked increase in eN/CD73 activity localized in gray (E) and white matter (F). After CTBS protocol (G), faint activity was observed in both gray (H) and white matter (I), similarly to control sections. Scale bar corresponds to 50 μm.