Figure 5.

The effects of hypoxia and the NF-κB signaling pathway on EMT in HCC cells. (A) EMT marker protein and cytokines expression in CoCl₂ induced HCC-LM3 and HepG2 cell lines were analyzed by Western blotting. IκBα, p-IκBα, total p65 (p65(T)) and nucleus p65 (p65(N)) expression levels in CoCl₂ stimulated HCC-LM3 cells were assessed. Quantification plots are presented on the right. GAPDH and Lamin B1 were used as control proteins. (B) HCC-LM3, SMMC-7721 and HepG2 cells were stimulated with 0, 10, and 20 ng/mL TNFα. IκBα, p-IκBα, p65 and p-p65 expressions were assessed by Western blot analysis. Quantification plots are shown below. (C) EMT maker protein and cytokines expression level were detected by Western blotting in TNFα-treated HCC-LM3, SMMC-7721 and HepG2 cells. Quantification plots are shown on the right. Data were shown as mean ± SD values (n = 3). * p < 0.05, ** p < 0.01 compared with untreated control cells. The figures shown were representative of three independent experiments.