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GCDCA in combination with poly(I:C) treatment of HIEs does not induce a greater type III IFN response. Jejunal HIEs were treated with 25 µg/mL poly(I:C) and increasing concentrations of GCDCA. At 24 h post treatment, mRNA levels of IFNL1 (shown), IFNL2, and IFNL3 were assayed. Previously unpublished data. Poly(I:C) treatment of HIEs and RT-qPCR for mRNA levels of type III IFNs performed as in Lin et al., 2020 [108].
