Figure 2.

PDMS-based MOC’s disposable unit fabrication. (a) A set of two compatible templates was 3D-printed, containing 4 coupled inlets and outlets: {1,2}, {3,4}, {5,6} and {7,8}. While ports {5,6} connect the cell culture compartments, the remaining ports enable controlled perfusion of fluid to each separate compartments, when ports {1,2}, {7,8} perfuse the two upper wells and ports {3,4} perfuse the lower well. (b) Before assembled two pieces of porous membrane (red arrows) were located on top of the wells’ template, so when connected (c) the pieces were locked between them (red arrows). PDMS was then poured into the hollow chamber through 4 ports and underwent overnight curing at 65 °C. (d) After peeling, the PDMS was punched and Plasma-bonded to a glass slide at its bottom side.