Figure 5.

MOC evaluation. (a) Disposable unit composed of 3D-printed top and bottom layers bonded using UV-based biocompatible (meth)acrylate resin. Red (top layer) and blue (bottom layer) colors stayed separated over a week which confirms the bonding efficiency. (b) The hydrophobic surface properties of the device result in a high contact angle of aqueous liquids, allowing the formation of spheroids using hanging drop cultures. (c) GFP-PC-9 cells confirmed the formation of spheroids within the device and was compared to spheroid formation in micro-mold-based methodology. Cell viability evaluated over 3 days (72 h) suggesting on the biocompatibility of the UV-based (meth)acrylate resin used. Scale bar: 500 µm and 300 µm for micro-mold-based spheroids and hanging drop-based spheroids respectively.