Figure 3.

In vitro growth kinetics in primary swine macrophage cell cultures for ASFV-G-∆KP177R and parental ASFV-G (MOI = 0.01). Samples were taken from three independent experiments at the indicated time points and titrated. Data represent means and standard deviations. Sensitivity using this methodology for detecting the virus is >log₁₀ 1.8 HAD₅₀/mL. No significant differences in viral yields between viruses were observed at any time point tested as determined using the Holm–Sidak method (α = 0.05), without assuming a consistent standard deviation. All calculations were conducted using Graphpad Prism software version 8.